Vitamin D(3) and methylenebisphosphonic acid in the correction of mineral metabolism disorders and bone remodeling associated with glucocorticoid-induced osteoporosis

The study was aimed at evaluating therapeutic efficacy of vitamin D3 (VD3, 1000 IU/kg b.w., 30 days) and sodium salt methylenebisphosphonic acid (MBPA, 17 mg/kg monotherapies as well their effect in combination preventing mineral metabolism bone remodeling disturbances associated with glucocorticoid(GC)-induced osteoporosis. Osteoporosis rats induced by long-term (30 administration the synthetic glucocorticoid prednisolone (5 b.w.). Calcium inorganic phosphate levels, activity alkaline phosphatase (ALP) serum, tissue marrow were determined spectrophotometrically. protein levels VD3 receptor (VDR), activator nuclear factor kappa-B (RANK), its ligand (RANKL), osteoprotegerin (OPG) Western blotting. Serum 25-hydroxyvitamin (25OHD3) content assayed ELISA. It shown that caused development hypocalcemia hypophosphatemia, increased blood while downregulating marrow. GC-induced osteoporosis accompanied a profound deficiency decrease VDR. Evaluation NF-κB-associated cytokine axis RANK/RANKL/OPG, which regulates balance osteoblasts/osteoclasts, showed simultaneous RANK OPG/RANKL ratio. Vitamin restored 25OHD3 level led to normalization VDR-mediated signaling­ RANK/RANKL/OPG functions tissue. has been MBPA had corrective on components serum tissue, activity­ only D3, indicating low efficiency bisphosphonate monotherapy Keywords: remode­ling, glucocorticoid-induced osteoporosis, acid, axis,